hydrochloric acid 109 057  (Merck & Co)

Journal: Scientific Reports

Article Title: Investigation of an oncolytic herpes simplex virus as a potential therapeutic agent for gastroenteropancreatic neuroendocrine neoplasms

doi: 10.1038/s41598-025-98588-7

Figure Lengend Snippet: QGP-1 and COA109 cells express HSV entry receptors. ( A ) The cell surface expression of viral entry receptors for M002 (CD111, CD112, syndecan, and HVEM) was assessed utilizing flow cytometry. QGP-1 (10 6 ) or COA109 (10 6 ) cells were cultured overnight in 6-well plates, collected, stained with fluorescent conjugated antibody, and evaluated with flow cytometry. Cells treated with FcR blocker alone served as negative controls. Receptors were expressed in variable quantities, but all four receptors were present on both cell types. ( B ) Representative histograms of flow cytometry for viral entry receptors in QGP-1 cells. Data represent three biologic replicates and are reported as mean ± standard error of the mean (SEM). * p ≤ 0.05, ** p ≤ 0.01, COA109 vs QGP-1.

Article Snippet: Briefly, phycoerythrin (PE) conjugated anti-human CD111 antibody (10 μL, Miltenyi Biotec), PE anti-human CD112 antibody (10 μL, Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (10 μL, Miltenyi Biotec), or APC anti-human HVEM (10 μL, Miltenyi Biotec), were added for 20 min on ice in the dark.

Techniques: Expressing, Flow Cytometry, Cell Culture, Staining

Journal: Translational Oncology

Article Title: Novel IL-15 dendritic cells have a potent immunomodulatory effect in immunotherapy of multiple myeloma

doi: 10.1016/j.tranon.2022.101413

Figure Lengend Snippet: Antibodies used in Flow cytometry analysis

Article Snippet: CD112-PE , R2.525 , Miltenyi Biotec (CA,USA).

Techniques: Flow Cytometry, Control, Staining

Journal: Translational Oncology

Article Title: Novel IL-15 dendritic cells have a potent immunomodulatory effect in immunotherapy of multiple myeloma

doi: 10.1016/j.tranon.2022.101413

Figure Lengend Snippet: IL-15 mDCs showed the enhanced cytotoxicity by generation of potent autologous cytotoxic T lymphocytes, CIK cells, and NK cells against primary autologous myeloma cells (paired). (A) We analyzed CD138 + primary myeloma cells isolated from bone marrow aspiration samples of newly diagnosed multiple myeloma patients to assess the expression levels of MHC class I, MICA, MICB, ULBP1, ULBP2/5/6, HLA-E, CD38 (n = 12), and CD112, CD155, ICAM-1 and PD-L1 (n = 5) using flow cytometry. ( B) We measured the expression of the degranulation marker CD107a in CTLs, CIK cells, and NK cells generated by mDCs against primary autologous myeloma cells. ( C) The killing capacity of activated lymphocytes by mDCs against primary autologous myeloma cells was investigated using an LDH-release cytotoxicity assay without or with anti-MHC class I mAb (n = 3). The data indicate that CLTs, CIK cells, and NK cells generated by the IL-15 mDCs (6 days) and IL-15 mDCs (8 days) groups exhibited stronger cytotoxicity than those of the conventional mDCs group. Data are representative from three independent experiments. *P < 0.05; ** P < 0.01; *** P < 0.001 (One-way ANOVA, multiple comparisons test: Tukey for B. Multiple t tests – one per row for C).

Article Snippet: CD112-PE , R2.525 , Miltenyi Biotec (CA,USA).

Techniques: Isolation, Expressing, Flow Cytometry, Marker, Generated, Cytotoxicity Assay